His-tag ELISA for the detection of humoral tumor-specific immunity
نویسندگان
چکیده
منابع مشابه
ELISA for detection of humoral immunity against measles
Measles is a highly infectious and contagious disease of the respiratory system caused by Morbilivirus (Hashiguchi et al., 2011). The clinical disease has been characterized by high fever , cough, conjunctivitis, coryza, malaise, maculopapular rash and erythematous patches throughout the body along with increased mortality among non-vaccinated children (Ellison, 1931; Fazlalipour et al., 2008)....
متن کاملSynthetics of NiFe2O4 nanoparticles for recombinant His-tag protein purification
Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...
متن کاملSynthetics of NiFe2O4 nanoparticles for recombinant His-tag protein purification
Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...
متن کاملHuman tumor cell lysates as a protein source for the detection of cancer antigen-specific humoral immunity.
Measurement of humoral tumor-specific immunity can predict what proteins are specific tumor antigens, be used to evaluate patient diagnosis or prognosis, and function as a method by which one can measure the effects of an immune intervention, such as a vaccine. Antibody assays can easily be adapted to high throughput formats; however, specific reagents needed for assay development often are not...
متن کاملSpecific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions.
Using a cell-bound immunogen, we have generated a monoclonal antibody, 3D5, that recognizes carboxy-terminal oligo-histidine tags (His tags) on a wide variety of proteins. From this monoclonal antibody, we have generated a single-chain fragment of the variable domains (scFv), a dimeric scFv-alkaline phosphatase fusion and an oligovalent scFv-display phage. The antibody in its various formats is...
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ژورنال
عنوان ژورنال: BMC Immunology
سال: 2008
ISSN: 1471-2172
DOI: 10.1186/1471-2172-9-23